L Glutamine Degradation Cell Culture

The rate and extent of l glutamine degradation are related to storage temperatures age of the product and ph.

L glutamine degradation cell culture.

L glutamine is also more labile in cell culture solutions than other amino acids. L glutamine is an unstable essential amino acid required in cell culture media formulations. In this study we examined the toxicity of ammonia for two different cell lines. According to sigma comment l glutamine is an unstable essential amino acid required in cell culture media formulations.

Among the lines in which gs activity is depressed by glutamine is a subclone of the hepatoma tissue culture htc cell line kulka and cohen 1973 which was used in this investigation. L glutamine participates in the formation of purine and pyrimidine nucleotides amino sugars glutathione l glutamate and other amino acids as well as in protein synthesis and glucose production. In ph 7 2 phosphate buffer and in two cell culture media the rate of the reaction was described by the same energy of activation 11 6 kcal mole for a temperature range of 4 to 37 c. Maintaining required levels of l glutamine in cell culture often requires the repeated supplementation of the medium with additional l glutamine.

L glutamine is one such essential component that can degrade in liquid medium and in culture. In aqueous solutions such as liquid culture media glutamine spontaneously decomposes into ammonia. This procedure can lead to toxic levels of ammonia build up as well as potential contamination. Most commercially available media are formulated with free l glutamine.

L glutamine is unstable at physiological ph in liquid media. Although l glutamine is an essential nutrient in cell cultures it spontaneously degrades in cell culture media generating ammonia and pyrrolidine carboxylic acid as byproducts. Most commercially available media are formulated with free l glutamine which is either included in the basal formula or added to liquid formulations at time of use. Thus at 37 c 10 per cent of the glutamine present decomposes per day while at 4 c a 10 per cent decomposition would take 9 days.

Gibco glutamax supplement is an improved cell culture supplement that can be used as a direct substitute for l glutamine in your cell culture media. Unlike most other amino acids l glutamine is not stable in solution. L glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L glutamine is an amino acid that is required for cell culture.