Irf3 Antibody Cell Signaling

Incubate membrane with anti rabbit igg hrp linked antibody 7074 at 1 2000 and anti biotin hrp linked antibody 7075 at 1 1000 1 3000 to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.

Irf3 antibody cell signaling.

Anti irf3 antibody epr2418y ab68481 at 1 1000 dilution lane 1. This induces a conformational change leading to its dimerization and nuclear localization. Anti irf3 phospho s386 antibody epr2346 ab76493 at 0 2 µg ml purified lane 1. Green ab68481 observed at 50 kda.

Anti phospho irak anti myd88 anti inhibitor of nf κb anti irf3 anti phospho stat1 anti stat1 anti erk1 2 anti p65 antibodies were purchased from cell signaling cytokine elisa kits were from r d systems and anti β actin antibody was from sigma aldrich. Irf3 interferon regulatory factor 3 is a protein coding gene. Merged signal red and green. Add primary antibody at the appropriate dilution as recommended in the product datasheet to 200 µl cell lysate at 1 mg ml.

It is phosphorylated by ikbke and tbk1 kinases. At 6 h subcellular localizations of irf3 and ago2 were visualized by immunofluorescence and microscopy. Mcf7 human breast adenocarcinoma epithelial cell whole cell lysates lane 2. 50kda and 33kda bands corresponding to irf3 isoforms were observed across all the cell lines.

Hela cell lysate lane 4. Incubate with rotation overnight at 4 c. Interferon regulatory factor 3 irf3 human. A8k7l2 b2raz3 q14653 q5fby1 q5fby2 q5fby4 q7z5g6.

A a549 cells were stimulated with sev for 6 h and cell proteins were extracted. B a549 cells were mock infected or infected with sev. The blot was probed with anti irf3 polyclonal antibody product pa5 20087 4 µg ml and detected by chemiluminescence using goat anti rabbit igg h l superclonal secondary antibody hrp conjugate product a27036 0 25 µg ml 1 4000 dilution. Wild type hap1 cell lysate lane 2.

Irf 3 irf3 interferon regulatory factor 3 immunogen. Mcf7 human breast adenocarcinoma epithelial cell treated with calyculin a for 45 minutes whole cell lysates. Anti irf3 antibody was used for immunoprecipitation. Diseases associated with irf3 include encephalopathy acute infection induced 7 and viral infectious disease among its related pathways are apoptosis modulation and signaling and activated tlr4 signalling gene ontology go annotations related to this gene include dna binding transcription factor activity and identical protein.

Lanes 1 4. 49 antibodies to irf3 and validated for use in 6 applications immunohistochemistry western blot flow cytometry chip assay immunocytochemistry elisa. Irf3 knockout hap1 cell lysate lane 3. Double stranded rna or toll like receptor signaling.

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding ser396 of human irf 3.