Human Nanog Antibody Cell Signaling

Hek 293 human embryonic kidney epithelial cell whole cell lysates with 5 nfdm tbst.

Human nanog antibody cell signaling.

Mouse embryonic stem cell line d3 lanes 4 5. Anti nanog antibody epr2027 2 ab109250 at 1 2000 dilution lane 1. Nanog expression is controlled by a network of factors including sox2 and the key pluripotency regulator oct 4 1. Nanog antibody gt3312 detects nanog protein by western blot analysis.

1 1000 the hrp conjugated anti mouse igg antibody gtx213111 01 was used to detect the primary antibody. Goat anti rabbit hrp at 1 2000 dilution developed using the ecl technique. Background nanog is a homeodomain containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells 1. Nccit human pluripotent embryonic carcinoma epithelial cell whole cell lysates with 5 nfdm tbst lane 2.

Marker lanes 2 3. Secondary all lanes. Nanog was detected in immersion fixed adlf1 top panel and fab2 bottom panel induced pluripotent stem cell lines using goat anti human nanog antigen affinity purified polyclonal antibody catalog af1997 at 10 µg ml for 3 hours at room temperature. Hela human cervix adenocarcinoma epithelial cell whole cell lysates with 5 nfdm tbst lane 3.

Anti nanog antibody ab80892 at 1 500 dilution lane 1. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human nanog protein. This antibody reacts with human samples. Clone rea314 recognizes the human nanog antigen a homeobox transcription factor also known as es cell associated protein 4.

Human nanog protein shares 52 overall amino acid identity with the mouse protein and 85 identity in the homeodomain. Tested in western blot wb immunofluorescence if immunocytochemistry icc and immunohistochemistry paraffin ihc p applications. Nanog mrna is present in pluripotent mouse and human cell lines and absent from differentiated cells. 30 μg human esc whole cell lysate extract 10 sds page nanog antibody gt3312 gtx627421 dilution.

2008 cell 132 1049 61. Nanog in adlf1 and fab2 stem cell lines. Invitrogen anti nanog monoclonal hnanog 2 ebioscience catalog 14 5768 82. Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog oct 4 sox2 klf4 c myc and lin28 2 3.

Supplied as 100 µg purified antibody 0 5 mg ml. Nanog is thought to be a key factor in maintaining pluripotency. Mutant mouse embryonic stem cell line known to not express nanog lysates proteins at 200000 cells per lane. The enriched dna was quantified by real time pcr using simplechip human oct 4 promoter primers 4641 simplechip human sox2 promoter primers 4649 and simplechip human α.

It is expressed in the central portion of the morula the inner cell mass of the blastocyst in embryonic stem cells esc and embryonic germ cells egc.