Human Mesenchymal Stem Cell Culture Protocol

Protocol for mesenchymal stem cell expansion mesenchymal stem cells mscs are currently widely used in clinical trials for cell therapy and regenerative medicine applications.

Human mesenchymal stem cell culture protocol.

Learn more about our hla. Whether mscs are used in basic research or in translational studies establishing the optimal mesenchymal stem cell expansion method and best performing medium allows. Our hmsc s are available from bone marrow adipose tissue and umbilical cord matrix of individual donors. Media and cells we offer everything you need for human mesenchymal stem cell culture.

Differentiation of human mesenchymal stem cells. This procedure uses 20 ml for each t75 flask used. When handling biohazard materials such as human cells safe laboratory procedures should be followed and protective clothing should be worn. Pre warm the completed stemxvivo msc expansion media in a 37 c water bath.

Osteogenesis of mesenchymal stem cells. Mesenchymal stem cell protocols download a free copy of our gibco mesenchymal stem cell sourcebook for a complete product reference guide product recommendations applications data and publications to support your msc workflow. Using a human fibronectin 20 µg ml or a 0 1 gelatin coated 48 well tissue culture plate seed 20k cells well in 0 5 ml normal msc growth media scm015 or scm045 after an overnight incubation replace the culture media with osteomax xf differentiation media. Coat a 6well tissue culture plate with 10 μg ml human fibronectin or bovine fibronectin according to the instruction manual.

Complete portfolio of primary human mesenchymal stem cells hmsc and optimized growth and differentiation media. Coat the culture vessel. Seed mesenchymal stem cells.