Human Mammary Epithelial Cells Culture Medium

Common uses of hmec include the study of breast cancer development three dimensional culture and carcinogen screening.

Human mammary epithelial cells culture medium.

Selected publications out of many where our customers have used megm growth media with various normal breast cells. Although all promocell media are optimized for use with primary human cells we have received feedback from customers that this particular medium can also be used for rat and murine mammary. Otherwise cells will not adhere well to the tissue culture flask. Atcc s human mammary epithelial cells cultured in mammary epithelial cell complete medium basal medium plus growth kit is an optimal serum free culture model for many research areas.

Human mammary epithelial cells in 2d culture. Presented at aacr 2017 we demonstrate here the feasibility of co culturing human mammary fibroblasts and human mammary epithelial cells in raft tm 3d culture system as a 3d breast cancer model. Enhanced growth of human mammary cells can be achieved by pre coating. This translates to greater experimental validity more reproducible results and reduction of lot to lot variability.

Our mammary epithelial cells are cryopreserved at passage 3 and can be passaged more than 4 times in our mammary epithelial cell culture medium. Mammary epithelial cells mepic lie within the alveolar lumen of the breast lobules which together with mammary ducts and adipose tissues form a complex network in the mammary gland. The promocell mammary epithelial cell growth medium is a serum free medium developed for the cultivation of epithelial cells from the mammary gland. Mammary epithelial cell basal medium contains essential and non essential amino acids vitamins other organic compounds trace minerals and inorganic salts.

Basal cells are marked by the expression of cytokeratin 5 green and luminal cells are marked by the expression of cytokeratin 18 red. Engineered specifically for culture of human mammary epithelial cells humec medium is a serum free formulation with frozen supplements containing growth factor and other components. Humec medium supports superior cell growth in the first culture after thaw and throughout the guaranteed lifespan when compared to a major competitors culture system figure 1. 3 0 culture of human mammary epithelial cells using epicult b or epicult c medium human human mammary epithelial cell cultures should be initiated from single cell suspensions refer to section 2 2.

Humec medium is a serum free medium engineered specifically for the culture of human mammary epithelial cells hmec. Primary human epithelial cells isolated from lung nasal mucosa trachea bronchi and distal respiratory tract and renal tissue whole kidney and renal cortex.